Structural Elucidation of Secondary Metabolites in Sponge (Callyspongia pseudoreticulata) with N-Hexane Extract

Structural Elucidation of Secondary Metabolites in Sponge (Callyspongia pseudoreticulata) with N-Hexane Extract

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Sponge is one Induction Cooktop of the marine species that has a high bioactivity and contains secondary metabobolite compound which can be used as antibiotic and medicine.Terpenoid compound derivatives have been successfully isolated from sponge Callyspongia pseudoretilulata by a method in which the n-hexane extract weighing 11.28 g was fractionated using vacuum column chromatography with a non-polar eluent, n-hexane.The polarity of this eluent was improved by ethyl acetic resulting in fairly polar eluent, methanol.

A total of Ladders 27 fractions were obtained and one of the compounds from n-hexane extract was structurally elucidated by NMR spectroscopy and two dimensions of homonuclear and heteronuclear (1H, 13C, DEPT, H-H COSY, HMQC, and HMBC).The identified compound was a hydrocarbon (1-ethyl cyclo hex cosana-1-amin) that indicated LC50 of 60.58 against A.salina and has a great potential as antitumor or anticancer.